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KMID : 0351619720130020223
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Kyungpook Medical Journal
1972 Volume.13 No. 2 p.223 ~ p.232
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Effects of Cortisone on Phagocytic Activity of Omental Milk Spots by Electron Microscopy
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Abstract
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It is well knon that the phagocy tic action of the cells of the reticuloendotheliIt is also known that when the reticuloendothelial system is blockaded by cortisone, adherence of the foreign bodies to the cell membrane of phagocytes, an initial step of phagocytic mechanism, and absorption of foreign body into the cytoplasm of phagocytes are considerably disturbed.
An investigation was undertaken to study t:he phagocytic activity of the omental milk spots by light and electron microscopy.
Sixteen albino rats, maintained on a stock diet, weighing 300.Ogm. average, were divided into 3 experimental groups.
Group 1. Control group.
No intraperitoneal injection of india ink given.
a. T~.vo rats, untreated.
b. 3 rats: A dose of 25 mg of cortisone acetate was given intramuscularly for 6 consecutive days.
Group 2.
6 rats: A single dose of 6.Oml of 2% india ink in normal saline ivas given once a day,. intraperitoneally, for 3 consecutive days.
Group 3.
6 rats: A dose of 35 mg of cortisone acetate was given intramuscularly, once a day, for 6 consecutive days. Starting on the 4th day> a 6.Oml of 2 o india ink in normal saline, once a day, was administered intraperitoneally, for 3 days.
Each cf the experimental rats were sacrificed 34 hours after the last treatment of india ink preparation free: the groups 3 and 3. The control group was sacrificed simillarly. Then the greater e~,~lentu:n teas extirpated and inspectedfor india ink pigmentation of the milk spots, followed by both light and electron micrescopic examinations.
The results were as follotivs:
1. Light microscopic observations:
In the experimental group of rats treated tivith 25 r.~g doses of cortisene acetate with a resulant reticuloendothelial system blockade follcwed by an intraperitoneal india ink injection (group 3;, the degree of carbon particle phagocytic activity of omental milk spots was greatly diminished over the group of rats treated with india-ink only (group 2).
2. Electron microscopic observations:
The number of phagosomes in the cytoplasms of the phagocytes of the milk spots of the greater ornentum was increased substantially in the group of rats treated with india ink only (group 2). In this group, a large quantity of carbon particles were found to be contained in the phagosomes.
Although there was an increased number of phagosomes bearing carbon particles in the group of rats treated with ´?5mg doses cortisone acetate followed by india ink injection (group 3), the number of phagosomes in this group vas less than in the group of rats treated with india ink alone (group 2). A considerable quantity of carbon particles were also found outside the cytoplasms of phagocytes for group 3.
It car. be concluded, therefore:
The phagocytic activity of omental milk spots ;eras greatly decimated but net completely inhibited n-hen the reticuloendothelizl system was blockaded by large quantity of cortisore administration.
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